Journal: Viruses

Article Title: Ephrin B1 and B2 Mediate Cedar Virus Entry into Egyptian Fruit Bat Cells

doi: 10.3390/v17040573

Figure Lengend Snippet: (q)PCR primers.

Article Snippet: Ephrin A2, A3, A4, B1 and B3 were synthesized at Eurofins Genomics, with the nucleotide sequences optimized according to human codon usage.

Techniques: Sequencing, Amplification

Journal: The Scientific World Journal

Article Title: Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

doi: 10.1155/2022/4567817

Figure Lengend Snippet: (a) LAMP primer design. Six distinct regions are designated on the target DNA, labeled F3, F2, F1, B1c, B2c, and B3 from the 5′ end. The F1c sequence is complementary to the F1 sequence. Two inner primers (FIP and BIP) and two outer primers (F3 and B3) are used in the LAMP method. FIP (BIP) is a hybrid primer consisting of the F1c (B1c) sequence and the F2 (B2) sequence. (b) LAMP primer mapping. The portion of the SopD gene that is targeted by the developed Salmonella enterica QUASR LAMP assay is shown here. The colored nitrogenous bases show the corresponding LAMP primer in .

Article Snippet: The assays were performed with 2.5 μ L sample DNA as a template in a total volume of 12.5 μ L containing 1.25 μ L 10 μ M Thermopol Buffer (New England BioLabs, Inc., Ipswich, MA, USA), 1.75 μ L 50 μ M MgSO 4 , 1.75 μ L 10 μ M dNTP (Promega, WI, USA), 1 μ L 5 M Betaine, 1.25 μ L 8 μ M Bst Polymerase (New England BioLabs, Inc., Ipswich, MA, USA), 0.5 μ L 40 μ M FIP and BIP primers (Macrogen, South Korea), 0.4 μ L 10 μ M F3 and B3 primers (Macrogen, South Korea), 0.5 μ L 60 μ M quencher probe (Macrogen, South Korea) and 0.7 μ L nuclease-free water.

Techniques: Labeling, Sequencing, Lamp Assay

Journal: The Scientific World Journal

Article Title: Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

doi: 10.1155/2022/4567817

Figure Lengend Snippet: In silico PCR amplification of SopD F3 and B3 primers. In silico PCR amplification was done against different bacterial genomes using a freely available molecular biology resource for experiments ( http://insilico.ehu.es/ ). Interpretation of results are as follows: (+) – Amplification was observed, (−) – No amplification was observed.

Article Snippet: The assays were performed with 2.5 μ L sample DNA as a template in a total volume of 12.5 μ L containing 1.25 μ L 10 μ M Thermopol Buffer (New England BioLabs, Inc., Ipswich, MA, USA), 1.75 μ L 50 μ M MgSO 4 , 1.75 μ L 10 μ M dNTP (Promega, WI, USA), 1 μ L 5 M Betaine, 1.25 μ L 8 μ M Bst Polymerase (New England BioLabs, Inc., Ipswich, MA, USA), 0.5 μ L 40 μ M FIP and BIP primers (Macrogen, South Korea), 0.4 μ L 10 μ M F3 and B3 primers (Macrogen, South Korea), 0.5 μ L 60 μ M quencher probe (Macrogen, South Korea) and 0.7 μ L nuclease-free water.

Techniques: In Silico, Amplification

Journal: Malawi Medical Journal

Article Title: The use of molecular technology to investigate trypanosome infections in tsetse flies at Liwonde Wild Life Reserve

doi: 10.4314/mmj.v31i4.3

Figure Lengend Snippet: List of primers used, their source, and expected product size

Article Snippet: The RIME-LAMP 25 μl reaction mixture contained 10 μl of lyophilised master mix containing dNTPs, Bsm polymerase, MgCl2, 5 μl of 5× reaction buffer (Thermo Scientific, USA), 1.0 μl of the outer forward primer (F3), 1.0 μl of the outer backward primer (B3), 1.0 μl of the forward inner primer (FIP), 1.0 μl of the backward inner primer (BIP), 1.0 μl of each of the loop forward and loop backward primers, LF & LB (Microsynth, Sweden), 2.0 μl of nuclease free water and 2.0 μl of template DNA.

Techniques: